Form/Appearance
N/A
Synonim names
N/A
Additional isotype
IgG
Other names
N/A
Gene synonims
N/A
Clonality
N/A
French translation
anticorps
Category
Antibodies
Conjugation
HRP Conjugated
Long name
HRP Donkey Anti-Mouse IgG (H+L)
Antigen
HRP Donkey Anti-Mouse IgG (H+L)
Latin name
Equus africanus asinus,Mus musculus
Gene name
HRP Donkey Anti-Mouse IgG (H+L) ; HRP
Clone
Not applicable to Polyclonal Antibodies
Applications
Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)
Concentration
Enquire us for detailed information on the concentration of this antibody
Specificity
This is an antibody designed to detect HRP Donkey Anti-Mouse IgG (H+L) ; HRP
Reacts with
Due to limitted amount of tested species we cannot guarantee that no crossreactivity will occur.
Purification method
The most common purification methods used in the antibody production are Affinity Chromatography, Caprylic Acid Ammonium Sulfate Precipitation, Antigen Affinity method, etc.
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.Antibody for research use.
Properties
If you buy Antibodies supplied by MBS Polyclonals they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.HRP conjugates are often primary rabbit polyclonal antibodies couples to the enzyme horseradish peroxidase (HRP), found in the roots of horseradish. Also monoclonal HRP conjugates are often used for Western Blot.
Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
About
Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.
Storage, shipping and handling
The antibody is shipped at +4 degrees Celsius. Upon receving, freeze at -20. For longer periods of time we recommend keeping the vial frozen at -40 or -80. Avoid cycles of freezing and thawing as they may denaturate the polypeptide chains of the antibody. Due to transportation or handling reasons, small amounts of the antibody might get caught on the lid or walls of the vial. We recommend you to briefly cetrifuge the vial prior to use to gather the content on the bottom.
