Synonim names
N/A
Other names
N/A
Gene synonims
N/A
Clonality
N/A
Clone
2C5
Conjugation
Flag
Gene name
Flag-Tag
Antigen
Flag-Tag
French translation
anticorps
Category
Antibodies
Concentration
100ug/100ul
Storage, shipping and handling
Store at -20 degree C for 1 year
Long name
Flag-Tag Mouse Monoclonal Antibody(2C5)
Applications
Western Blot (WB), Immunoprecipitation (IP), Immunofluorescence (IF)
Reacts with
Due to limitted amount of tested species we cannot guarantee that no crossreactivity will occur.
Form/Appearance
Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Specificity
The Flag tag antibody can recognize C-terminal, internal, and N-terminal Flag-tag fusion proteins. ;This is an antibody designed to detect Flag-Tag
Purification method
The most common purification methods used in the antibody production are Affinity Chromatography, Caprylic Acid Ammonium Sulfate Precipitation, Antigen Affinity method, etc.
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.Antibody for research use.
Properties
If you buy Antibodies supplied by MBS Polyclonals they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.An anti-flag tag (FLAG fusion protein) is use to detect a FLAG-tag, or FLAG octapeptide, or FLAG epitope that is a polypeptide protein tag that can be added to a protein using recombinant DNA. This FLAG-tags have the sequence DYKDDDDK motiv. These tags are very useful to do protein purification by affinity chromatography. Also separation of recombinant, overexpressed proteins from cell lysates is done by FLAG go HIS tags. FLAGS are also used in the isolation of protein complexes with multiple subunits, because its mild purification procedure tends not to disrupt such complexes. It has been used to enrich proteins of height purity and quality to see the 3D crystal structure with x-ray. Suitable for in vivo use in cells. For electrophorese protein detection rabbit polyclonals anti Flag conjugation are the most suited antibodies.
