Other names
N/A
Gene synonims
N/A
Form/Appearance
N/A
Conjugation
Flag
Concentration
1.4mg/mL
Antigen
FLAG tag
French translation
anticorps
Category
Antibodies
Gene name
FLAG tag ; FLAG
Clonality
Polyclonal Antibodies
Long name
FLAG tag Polyclonal Antibody
Clone
Not applicable to Polyclonal Antibodies
Synonim names
DDDDK epitope tag; DYKDDDDK epitope tag
Reacts with
Fusion proteins containing the Flag Tag
Applications
ELISA, EIA, IFA, ELI-Spot, Western Blot (WB)
Specificity
This is an antibody designed to detect FLAG tag ; FLAG
Purification method
This antibody was purified via antigen affinity purification method
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.Antibody for research use.
Storage, shipping and handling
The antibody is shipped at +4 degrees Celsius. Upon receving, freeze at -20. For longer periods of time we recommend keeping the vial frozen at -40 or -80. Avoid cycles of freezing and thawing as they may denaturate the polypeptide chains of the antibody. Due to transportation or handling reasons, small amounts of the antibody might get caught on the lid or walls of the vial. We recommend you to briefly cetrifuge the vial prior to use to gather the content on the bottom.
Properties
If you buy Antibodies supplied by MBS Polyclonals they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.An anti-flag tag (FLAG fusion protein) is use to detect a FLAG-tag, or FLAG octapeptide, or FLAG epitope that is a polypeptide protein tag that can be added to a protein using recombinant DNA. This FLAG-tags have the sequence DYKDDDDK motiv. These tags are very useful to do protein purification by affinity chromatography. Also separation of recombinant, overexpressed proteins from cell lysates is done by FLAG go HIS tags. FLAGS are also used in the isolation of protein complexes with multiple subunits, because its mild purification procedure tends not to disrupt such complexes. It has been used to enrich proteins of height purity and quality to see the 3D crystal structure with x-ray. Suitable for in vivo use in cells. For electrophorese protein detection rabbit polyclonals anti Flag conjugation are the most suited antibodies.
