Synonim names
N/A
Concentration
0.2mg/ml
French translation
anticorps
Category
Antibodies
Tissue
cell, prostate, stem
Clonality
Polyclonal Antibodies
Gene synonims
PSCA; PSCA; PRO232; N/A
Antigen
Prostate Stem Cell Antigen (PSCA)
Clone
Not applicable to Polyclonal Antibodies
Gene name
Prostate Stem Cell Antigen (PSCA) ; PSCA
Purification method
This antibody was purified via Affinity Chromatography
Long name
Biotin-Linked Antibody to Prostate Stem Cell Antigen (PSCA)
Applications
Immunohistochemistry (IHC), ELISA, EIA, IFA, ELI-Spot, Western Blot (WB)
Form/Appearance
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
Reacts with
Due to limitted amount of tested species we cannot guarantee that no crossreactivity will occur.
Other names
prostate stem cell antigen preproprotein; Prostate stem cell antigen; prostate stem cell antigen; prostate stem cell antigen; N/A
Properties
If you buy Antibodies supplied by MBS Polyclonals they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
Specificity
The antibody is a rabbit polyclonal antibody raised against PSCA conjugated to biotin. It has been selected for its ability to recognize PSCA in immunohistochemical staining andwestern blotting. ;This is an antibody designed to detect Prostate Stem Cell Antigen (PSCA) ; PSCA
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.Antibody for research use.Antigens are peptides or recombinant or native dependent on the production method.For cells, cell lines and tissues in culture till half confluency.
Storage, shipping and handling
The antibody is shipped at +4 degrees Celsius. Upon receving, freeze at -20. For longer periods of time we recommend keeping the vial frozen at -40 or -80. Avoid cycles of freezing and thawing as they may denaturate the polypeptide chains of the antibody. Due to transportation or handling reasons, small amounts of the antibody might get caught on the lid or walls of the vial. We recommend you to briefly cetrifuge the vial prior to use to gather the content on the bottom.
Test
Stem cell factors and stem cell growth factors will produce stem cells or be part of a transdifferentiation process to produce other cells. A cell can transdifferentiate by going back to the naive stem cell stadium or directly into the other cell, helped by the stem cell and transdifferentiationf actors. Stem cell growth factors or stem cell factors are mostly used to produce iPSCs or induced pluripotent stem cells by Jamaka or Thomson factors by using for example 5 Lenti-III-CMV viruses, expressing the Yamanaka iPSC factor set (Oct4, Sox2, Nanog and Lin28) + GFP positive control. Trans differentiation will omit the stem cell stadium but stem cell factors sill play an important role in trans differentiation strategies.