Mouse monoclonal-Anti- Fluorescein Isothiocyanate (FITC)-20ug

Size

20ug

Catalog no#

MAO843Ge21-20ug

Price

256 EUR

Image number

-

Sequence of immunogen

-

Concentration

1mg/ml

Alternative Names

FITC-Tag

Species reactivity

Pan-species

Latin name

Mus musculus

Clonality

Mouse monoclonal

Aplication

WB; IHC; ICC; IP.

Delivery condition

4℃ with ice bags

Conjugation

Anti-FITC Antibody

Organism Species

Pan-species (General)

Item Name

Fluorescein Isothiocyanate

Source

Monoclonal antibody preparation

Purification

Protein A + Protein G affinity chromatography

Buffer Formulation

PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.

Immunogen

CPO843Ge21-OVA Conjugated Fluorescein Isothiocyanate (FITC)

Applicable Secondary Antibody

SAA544Mu08, SAA544Mu09, SAA544Mu07, SAA544Mu19, SAA544Mu18, SAA544Mu17

Storage instructions

Avoid repeated freeze/thaw cycles. Store at 4 ℃ for frequent use. Aliquot and store at -20℃ for 12 months.

Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.Antibody for research use.

About

Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.

Test

Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.

Properties

This Cloud Clone Corp Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Cloud Clone Corp FITC is highly sensitive to pH extremes.