Image number
-
Sequence of immunogen
-
Concentration
1mg/ml
Alternative Names
FITC-Tag
Species reactivity
Pan-species
Clonality
Mouse monoclonal
Aplication
WB; IHC; ICC; IP.
Conjugation
Anti-FITC Antibody
Delivery condition
4℃ with ice bags
Organism Species
Pan-species (General)
Item Name
Fluorescein Isothiocyanate
Source
Monoclonal antibody preparation
Purification
Protein A + Protein G affinity chromatography
Buffer Formulation
PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Immunogen
CPO843Ge21-OVA Conjugated Fluorescein Isothiocyanate (FITC)
Applicable Secondary Antibody
SAA544Mu08, SAA544Mu09, SAA544Mu07, SAA544Mu19, SAA544Mu18, SAA544Mu17
Storage instructions
Avoid repeated freeze/thaw cycles. Store at 4 ℃ for frequent use. Aliquot and store at -20℃ for 12 months.
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.Antibody for research use.
Properties
This Cloud Clone Corp Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Cloud Clone Corp FITC is highly sensitive to pH extremes.
