Image number
1
Sequence of immunogen
-
Aplication
WB
Tissue
cell
Species reactivity
Human
Concentration
1mg/ml
French translation
anticorps
Latin name
Mus musculus
Clonality
Mouse monoclonal
Delivery condition
4℃ with ice bags
Organism Species
Homo sapiens (Human)
Alternative Names
CD31; EndoCAM; GPIIA'; PECA1
Source
Monoclonal antibody preparation
Item Name
Platelet/Endothelial Cell Adhesion Molecule
Purification
Protein A + Protein G affinity chromatography
Buffer Formulation
0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
Applicable Secondary Antibody
SAA544Mu08, SAA544Mu09, SAA544Mu07, SAA544Mu19, SAA544Mu18, SAA544Mu17
Immunogen
RPA363Hu01-Recombinant Platelet/Endothelial Cell Adhesion Molecule (PECAM1)
Storage instructions
Avoid repeated freeze/thaw cycles. Store at 4 ℃ for frequent use. Aliquot and store at -20℃ for 12 months.
Properties
If you buy Antibodies supplied by Cloud Clone Corp they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
About
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
Description
cell adhesion molecules play a role in cell growth and activation and are often identified by WB or ELISA as in the Mouse, Anti- Platelet/Endothelial Adhesion Molecule (PECAM1)-Monoclonal antibody.This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.Antibody for research use.For cells, cell lines and tissues in culture till half confluency.Whole adhesion and interacting molecules are present in lysates used as reference for ELISA quantification of these molecules and their subunits.Platelets, also called thrombocytes or cloth cells in blood and are needed to stop bleeding by clumping and clotting the blood the vessels when the an injury occurs. Teh bone marrow will produce the platelets that have no nucleus. Platelates are unique to mammals, the are curved shaped 1900nm to 3100 nm large nucleus free clothing structures.