Clone:
RM212
Antibody host:
Rabbit
Form of antibody:
Liquid
Concentration of antibody:
1 mg/ml
Target of this antibody:
HIST1H4A
Shipping conditions:
gel pack
French translation
anticorps
Isotype:
Rabbit IgG
Clonality of antibody:
Monoclonal
This antibody reacts with:
Human, mouse, rat
Latin name
Oryctolagus cuniculus
This antibody has been succesfully tested for use in following methods:
WB, ICC, ELISA, Multiplex
Purification method:
Affinity purified using protein A
Alternative names of antibody target:
H4FB, HIST1H4B, HIST1H4F, H4FN, H4FH
Storage information:
Shelf time: 12 months in temp.: -20 deg.C
Formulation of this antibody:
In 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
How to handle this antibody:
The antibody solution should be gently mixed before use.
Immunogene sequence:
A peptide corresponding to the C-terminus of human Histone H4
Recommended usage:
Western Blot: 0.1 ug/ml – 0.5 ug/mlICC: 1 μg/ml - 2 μg/mlELISA: 0.2 ug/ml - 1 ug/mlMultiplex: 0.1 ug/ml – 0.5 ug/ml
Properties
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.Antibody for research use.
Background information:
The nucleosome is made up of four core histone proteins (H2A, H2B, H3 and H4) and is the primary building block of chromatin. The N-terminal tail of core histones undergoes different posttranscriptional modification including acetylation, phosphorylation and methylation. These modifications occur in response to cell signal stimuli and have a direct effect on gene expression.
About
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.Rabbits are used for polyclonal antibody production by Biovision. Rabbit antibodies are very stable and can be stored for several days at room temperature. Biovision adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
