Target of this antibody:
H3F3A
Clone:
RM190
This antibody reacts with:
Human
Antibody host:
Rabbit
Form of antibody:
Liquid
Concentration of antibody:
1 mg/ml
Shipping conditions:
gel pack
French translation
anticorps
Isotype:
Rabbit IgG
Clonality of antibody:
Monoclonal
This antibody has been succesfully tested for use in following methods:
WB, ELISA, Multiplex
Latin name
Oryctolagus cuniculus
Alternative names of antibody target:
H3F3A, H3.3A, H3F3, H3F3B, H3.3B
Purification method:
Affinity purified using protein A
Storage information:
Shelf time: 12 months in temp.: -20 deg.C
Immunogene sequence:
A peptide corresponding to human Histone H3.3.
Formulation of this antibody:
In 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
How to handle this antibody:
The antibody solution should be gently mixed before use.
Recommended usage:
Western Blot: 0.5 ug/mL – 2 ug/mL; ELISA: 0.2 ug/mL - 1 ug/mL; Multiplex: 0.05 ug/mL – 0.5 ug/mL.
Properties
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.Antibody for research use.
About
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.Rabbits are used for polyclonal antibody production by Biovision. Rabbit antibodies are very stable and can be stored for several days at room temperature. Biovision adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
Background information:
Variant histone H3 which replaces conventional H3 in a wide range of nucleosomes in active genes. Constitutes the predominant form of histone H3 in non-dividing cells and is incorporated into chromatin independently of DNA synthesis. Deposited at sites of nucleosomal displacement throughout transcribed genes, suggesting that it represents an epigenetic imprint of transcriptionally active chromatin. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
