anti-Anti- Histone H3.3 Rabbit Monoclonal Antibody

Size

Anti- Histone H3.3 Rabbit Monoclonal Antibody

Catalog no#

A1140-25

Price

262 EUR

Target of this antibody:

H3F3A

Clone:

RM190

This antibody reacts with:

Human

Antibody host:

Rabbit

Form of antibody:

Liquid

Concentration of antibody:

1 mg/ml

Shipping conditions:

gel pack

French translation

anticorps

Isotype:

Rabbit IgG

Clonality of antibody:

Monoclonal

This antibody has been succesfully tested for use in following methods:

WB, ELISA, Multiplex

Latin name

Oryctolagus cuniculus

Alternative names of antibody target:

H3F3A, H3.3A, H3F3, H3F3B, H3.3B

Purification method:

Affinity purified using protein A

Storage information:

Shelf time: 12 months in temp.: -20 deg.C

Immunogene sequence:

A peptide corresponding to human Histone H3.3.

Formulation of this antibody:

In 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide

How to handle this antibody:

The antibody solution should be gently mixed before use.

Recommended usage:

Western Blot: 0.5 ug/mL – 2 ug/mL; ELISA: 0.2 ug/mL - 1 ug/mL; Multiplex: 0.05 ug/mL – 0.5 ug/mL.

Properties

If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.

Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.Antibody for research use.

About

Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.Rabbits are used for polyclonal antibody production by Biovision. Rabbit antibodies are very stable and can be stored for several days at room temperature. Biovision adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.

Background information:

Variant histone H3 which replaces conventional H3 in a wide range of nucleosomes in active genes. Constitutes the predominant form of histone H3 in non-dividing cells and is incorporated into chromatin independently of DNA synthesis. Deposited at sites of nucleosomal displacement throughout transcribed genes, suggesting that it represents an epigenetic imprint of transcriptionally active chromatin. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.