Uniprot ID
P31749
Calculated MW
60 kDa
Form
liquid
French translation
anticorps
Specificity
Human, Mouse, Rat
Tested Application
ELISA, WB, IHC, IF
Purification
Immunogen affinity purified
Purity
≥95% as determined by SDS-PAGE
Immunogen
v-akt murine thymoma viral oncogene homolog 1
Recommended dilution
WB: 1:500 - 1:2000; IHC: 1:50 - 1:100; IF: 1:50 - 1:100
Synonyms
AKT, AKT1, PKB, PKB ALPHA, PRKBA, Protein kinase B, Proto oncogene c Akt, RAC, RAC ALPHA, RAC PK alpha
Image1
Immunohistochemistry of paraffin-embedded human ovary tumor using FNab00268(AKT antibody) at dilution of 1:50
Image4
HeLa cells were subjected to SDS PAGE followed by western blot with FNab00268(AKT antibody) at dilution of 1:1000
Storage
PBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20℃ for 24 months (Avoid repeated freeze / thaw cycles.)
Properties
If you buy Antibodies supplied by FineTest they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.Antibody for research use.
Background
The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Mutations in this gene have been associated with the Proteus syndrome. Multiple alternatively spliced transcript variants have been found for this gene.
