Specificity
Human
Uniprot ID
P27695
Form
liquid
French translation
anticorps
Calculated MW
Refer to figures
Tested Application
ELISA, WB, IP, IF
Purification
Immunogen affinity purified
Synonyms
APE, APE1, APEX, APX, HAP1, REF1
Purity
≥95% as determined by SDS-PAGE
Immunogen
APEX nuclease (multifunctional DNA repair enzyme) 1
Recommended dilution
WB: 1:500 - 1:2000; IF: 1:50 - 1:100; IP: 1:50 - 1:200
Storage
PBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20℃ for 24 months (Avoid repeated freeze / thaw cycles.)
Image1
Immunohistochemistry of paraffin-embedded human cervical cancer using FNab00479(APEX1 antibody) at dilution of 1:50
Image4
HepG2 cells were subjected to SDS PAGE followed by western blot with FNab00479(APEX1 antibody) at dilution of 1:1000
Properties
If you buy Antibodies supplied by FineTest they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.Antibody for research use.
Background
Apurinic/apyrimidinic (AP) sites occur frequently in DNA molecules by spontaneous hydrolysis, by DNA damaging agents or by DNA glycosylases that remove specific abnormal bases. AP sites are pre-mutagenic lesions that can prevent normal DNA replication so the cell contains systems to identify and repair such sites. Class II AP endonucleases cleave the phosphodiester backbone 5' to the AP site. This gene encodes the major AP endonuclease in human cells. Splice variants have been found for this gene; all encode the same protein.
