anti- APEX1 antibody

Size

2X100μg

Catalog no#

FNab00479

Price

320 EUR

Specificity

Human

Uniprot ID

P27695

Form

liquid

French translation

anticorps

Calculated MW

Refer to figures

Tested Application

ELISA, WB, IP, IF

Purification

Immunogen affinity purified

Synonyms

APE, APE1, APEX, APX, HAP1, REF1

Purity

≥95% as determined by SDS-PAGE

Immunogen

APEX nuclease (multifunctional DNA repair enzyme) 1

Recommended dilution

WB: 1:500 - 1:2000; IF: 1:50 - 1:100; IP: 1:50 - 1:200

Storage

PBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20℃ for 24 months (Avoid repeated freeze / thaw cycles.)

Image1

Immunohistochemistry of paraffin-embedded human cervical cancer using FNab00479(APEX1 antibody) at dilution of 1:50

Image4

HepG2 cells were subjected to SDS PAGE followed by western blot with FNab00479(APEX1 antibody) at dilution of 1:1000

Properties

If you buy Antibodies supplied by FineTest they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.

Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.Antibody for research use.

Background

Apurinic/apyrimidinic (AP) sites occur frequently in DNA molecules by spontaneous hydrolysis, by DNA damaging agents or by DNA glycosylases that remove specific abnormal bases. AP sites are pre-mutagenic lesions that can prevent normal DNA replication so the cell contains systems to identify and repair such sites. Class II AP endonucleases cleave the phosphodiester backbone 5' to the AP site. This gene encodes the major AP endonuclease in human cells. Splice variants have been found for this gene; all encode the same protein.