Specificity
Human
Calculated MW
70 kDa
Uniprot ID
P42331
Form
liquid
Synonyms
KIAA0053
French translation
anticorps
Tested Application
ELISA, WB, IHC
Purification
Immunogen affinity purified
Immunogen
Rho GTPase activating protein 25
Purity
≥95% as determined by SDS-PAGE
Recommended dilution
WB: 1:500 - 1:2000; IHC: 1:50 - 1:200
Storage
PBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20℃ for 24 months (Avoid repeated freeze / thaw cycles.)
Image4
Jurkat cells were subjected to SDS PAGE followed by western blot with FNab00551(ARHGAP25 antibody) at dilution of 1:1000
Image1
Immunohistochemistry of paraffin-embedded human tonsillitis tissue slide using FNab00551( ARHGAP25 Antibody) at dilution of 1:200
Properties
If you buy Antibodies supplied by FineTest they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.Antibody for research use.
Background
ARHGAPs, such as ARHGAP25, encode negative regulators of Rho GTPases (see ARHA; MIM 165390), which are implicated in actin remodeling, cell polarity, and cell migration (Katoh and Katoh, 2004 [PubMed 15254788]).