Uniprot ID
P10415
Calculated MW
26 kDa
Form
liquid
French translation
anticorps
Tested Application
ELISA, WB, IHC
Specificity
Human, Mouse, Rat
Immunogen
B-cell CLL/lymphoma 2
Purification
Immunogen affinity purified
Purity
≥95% as determined by SDS-PAGE
Recommended dilution
WB: 1:500 - 1:2000; IHC: 1:50 - 1:100
Synonyms
Apoptosis regulator Bcl 2, B cell CLL/lymphoma 2, BCL2, Oncogene B cell leukemia 2
Storage
PBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20℃ for 24 months (Avoid repeated freeze / thaw cycles.)
Image4
HL-60 cells were subjected to SDS PAGE followed by western blot with FNab00839( Bcl2 Antibody) at dilution of 1:1000
Properties
If you buy Antibodies supplied by FineTest they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
Image1
Immunohistochemistry of paraffin-embedded human tonsillitis tissue slide using FNab00839( BCL2 Antibody) at dilution of 1:200 . heat mediated antigen retrieved with Tris-EDTA buffer(pH9).
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.Antibody for research use.
Background
This gene encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Alternative splicing results in multiple transcript variants.
