Uniprot ID
P49711
Form
liquid
Calculated MW
135 kDa
French translation
anticorps
Specificity
Human, Mouse, Rat
Tested Application
ELISA, WB, IHC, IP
Purification
Immunogen affinity purified
Purity
≥95% as determined by SDS-PAGE
Immunogen
CCCTC-binding factor (zinc finger protein)
Recommended dilution
WB: 1:1000 - 1:2000; IHC: 1:50 - 1:200; ChIP : 1:50 - 1:200
Image1
Immunohistochemistry of paraffin-embedded human lymphoma using FNab02049(CTCF antibody) at dilution of 1:100
Storage
PBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20℃ for 24 months (Avoid repeated freeze / thaw cycles.)
Image4
human brain tissue were subjected to SDS PAGE followed by western blot with FNab02049(CTCF antibody) at dilution of 1:1000
Properties
If you buy Antibodies supplied by FineTest they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.Antibody for research use.
Background
This gene is a member of the BORIS + CTCF gene family and encodes a transcriptional regulator protein with 11 highly conserved zinc finger (ZF) domains. This nuclear protein is able to use different combinations of the ZF domains to bind different DNA target sequences and proteins. Depending upon the context of the site, the protein can bind a histone acetyltransferase (HAT)-containing complex and function as a transcriptional activator or bind a histone deacetylase (HDAC)-containing complex and function as a transcriptional repressor. If the protein is bound to a transcriptional insulator element, it can block communication between enhancers and upstream promoters, thereby regulating imprinted expression. Mutations in this gene have been associated with invasive breast cancers, prostate cancers, and Wilms' tumors. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
