Uniprot ID
P00533
Form
liquid
Calculated MW
160 kDa
French translation
anticorps
Tested Application
ELISA, WB, IHC
Specificity
Human, Mouse, Rat
Purification
Immunogen affinity purified
Purity
≥95% as determined by SDS-PAGE
Recommended dilution
WB: 1:500 - 1:2000; IHC: 1:50 - 1:200
Synonyms
EGFR, ERBB, ERBB1, HER1, mENA, PIG61, Proto oncogene c ErbB 1
Immunogen
epidermal growth factor receptor (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian)
Storage
PBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20℃ for 24 months (Avoid repeated freeze / thaw cycles.)
Image4
A431 cells were subjected to SDS PAGE followed by western blot with FNab02667( EGFR Antibody) at dilution of 1:1000
Image1
Immunohistochemistry of paraffin-embedded human lung cancer slide using FNab02667( EGFR Antibody) at dilution of 1:50
Properties
If you buy Antibodies supplied by FineTest they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.Antibody for research use.
Background
The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer.