Uniprot ID
P00533
Form
liquid
Calculated MW
180 kDa
French translation
anticorps
Specificity
Human, Mouse, Rat
Synonyms
ERBB, ERBB1, HER1
Tested Application
ELISA, WB, IHC, IP, IF
Purification
Immunogen affinity purified
Purity
≥95% as determined by SDS-PAGE
Recommended dilution
WB: 1:500 - 1:2000; IHC: 1:50 - 1:200
Immunogen
epidermal growth factor receptor (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian)
Storage
PBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20℃ for 24 months (Avoid repeated freeze / thaw cycles.)
Image4
A431 cells were subjected to SDS PAGE followed by western blot with FNab02668( EGFR-Specific Antibody) at dilution of 1:1000
Properties
If you buy Antibodies supplied by FineTest they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
Image1
Immunofluorescent analysis of Hela cells, using FNab02668( EGFR antibody ) at 1:25 dilution and Rhodamine-labeled goat anti- rabbit IgG (red).
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.Antibody for research use.
Background
The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer.
