Specificity
Human
Uniprot ID
Q04637
Form
liquid
Calculated MW
200 kDa
French translation
anticorps
Tested Application
ELISA, WB, IHC, IF
Synonyms
EIF4F, EIF4G, EIF4GI
Purification
Immunogen affinity purified
Purity
≥95% as determined by SDS-PAGE
Immunogen
eukaryotic translation initiation factor 4 gamma, 1
Recommended dilution
WB: 1:500 - 1:2000; IHC: 1:50 - 1:200; IF: 1:50 - 1:200
Storage
PBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20℃ for 24 months (Avoid repeated freeze / thaw cycles.)
Image1
Immunohistochemistry of paraffin-embedded human breast cancer using FNab02724(EIF4G1 antibody) at dilution of 1:100
Image4
HeLa cells were subjected to SDS PAGE followed by western blot with FNab02724(EIF4G1 antibody) at dilution of 1:1000
Properties
If you buy Antibodies supplied by FineTest they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.Antibody for research use.
Background
The protein encoded by this gene is a component of the multi-subunit protein complex EIF4F. This complex facilitates the recruitment of mRNA to the ribosome, which is a rate-limiting step during the initiation phase of protein synthesis. The recognition of the mRNA cap and the ATP-dependent unwinding of 5'-terminal secondary structure is catalyzed by factors in this complex. The subunit encoded by this gene is a large scaffolding protein that contains binding sites for other members of the EIF4F complex. A domain at its N-terminus can also interact with the poly(A)-binding protein, which may mediate the circularization of mRNA during translation. Alternative splicing results in multiple transcript variants, some of which are derived from alternative promoter usage.
