anti- ERK1/2 antibody

Size

2X100μg

Catalog no#

FNab02845

Price

320 EUR

Uniprot ID

P28482

Calculated MW

40 kDa

Form

liquid

French translation

anticorps

Tested Application

ELISA, WB, IHC

Specificity

Human, Mouse, Rat

Purification

Immunogen affinity purified

Purity

≥95% as determined by SDS-PAGE

Immunogen

mitogen-activated protein kinase 1

Recommended dilution

WB: 1:500 - 1:2000; IHC: 1:50 - 1:100

Storage

PBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20℃ for 24 months (Avoid repeated freeze / thaw cycles.)

Image4

HeLa cells were subjected to SDS PAGE followed by western blot with FNab02845(ERK1/2 antibody) at dilution of 1:1000

Properties

If you buy Antibodies supplied by FineTest they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.

Synonyms

ERK, ERK 2, ERK1/2, ERK2, ERT1, MAP kinase 1, MAP kinase 2, MAP kinase isoform p42, MAPK 1, MAPK 2, MAPK1, MAPK2, p40, p41, p41mapk, p42 MAPK, P42MAPK, PRKM1, PRKM2

Image1

Immunohistochemistry of paraffin-embedded human colon cancer tissue slide using FNab02845( ERK1/2 Antibody) at dilution of 1:200 heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.Antibody for research use.

Background

This gene encodes a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. The activation of this kinase requires its phosphorylation by upstream kinases. Upon activation, this kinase translocates to the nucleus of the stimulated cells, where it phosphorylates nuclear targets. One study also suggests that this protein acts as a transcriptional repressor independent of its kinase activity. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Two alternatively spliced transcript variants encoding the same protein, but differing in the UTRs, have been reported for this gene.