anti- GALE antibody

Size

2X100μg

Catalog no#

FNab03309

Price

320 EUR

Uniprot ID

Q14376

Calculated MW

36 kDa

Form

liquid

French translation

anticorps

Tested Application

ELISA, WB, IHC

Specificity

Human, Mouse, Rat

Immunogen

UDP-galactose-4-epimerase

Purification

Immunogen affinity purified

Purity

≥95% as determined by SDS-PAGE

Recommended dilution

WB: 1:500 - 1:2000; IHC: 1:50 - 1:200; IF: 1:10 - 1:100

Storage

PBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20℃ for 24 months (Avoid repeated freeze / thaw cycles.)

Image4

A375 cells were subjected to SDS PAGE followed by western blot with FNab03309(GALE antibody) at dilution of 1:1000

Image1

Immunohistochemistry of paraffin-embedded human malignant melanoma using FNab03309(GALE antibody) at dilution of 1:100

Properties

If you buy Antibodies supplied by FineTest they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.

Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.Antibody for research use.

Background

This gene encodes UDP-galactose-4-epimerase which catalyzes two distinct but analogous reactions: the epimerization of UDP-glucose to UDP-galactose, and the epimerization of UDP-N-acetylglucosamine to UDP-N-acetylgalactosamine. The bifunctional nature of the enzyme has the important metabolic consequence that mutant cells (or individuals) are dependent not only on exogenous galactose, but also on exogenous N-acetylgalactosamine as a necessary precursor for the synthesis of glycoproteins and glycolipids. Mutations in this gene result in epimerase-deficiency galactosemia, also referred to as galactosemia type 3, a disease characterized by liver damage, early-onset cataracts, deafness and mental retardation, with symptoms ranging from mild ('peripheral' form) to severe ('generalized' form). Multiple alternatively spliced transcripts encoding the same protein have been identified.