Uniprot ID
P50135
Calculated MW
27 kDa
Form
liquid
French translation
anticorps
Specificity
Human, Mouse
Tested Application
ELISA, WB, IHC, IP
Purification
Immunogen affinity purified
Immunogen
histamine N-methyltransferase
Purity
≥95% as determined by SDS-PAGE
Recommended dilution
WB: 1:500 - 1:2000; IHC: 1:50 - 1:200; IP : 1:20 - 1:50
Image1
Immunohistochemistry of paraffin-embedded human lymphoma using FNab03944(HNMT antibody) at dilution of 1:50
Storage
PBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20℃ for 24 months (Avoid repeated freeze / thaw cycles.)
Image4
human liver tissue were subjected to SDS PAGE followed by western blot with FNab03944(HNMT antibody) at dilution of 1:1000
Properties
If you buy Antibodies supplied by FineTest they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.Antibody for research use.
Background
In mammals, histamine is metabolized by two major pathways: N(tau)-methylation via histamine N-methyltransferase and oxidative deamination via diamine oxidase. This gene encodes the first enzyme which is found in the cytosol and uses S-adenosyl-L-methionine as the methyl donor. In the mammalian brain, the neurotransmitter activity of histamine is controlled by N(tau)-methylation as diamine oxidase is not found in the central nervous system. A common genetic polymorphism affects the activity levels of this gene product in red blood cells. Multiple alternatively spliced transcript variants that encode different proteins have been found for this gene.