Uniprot ID
Q14145
Calculated MW
85 kDa
Form
liquid
French translation
anticorps
Specificity
Human, Mouse, Rat
Tested Application
ELISA, IHC, IF, WB
Purification
Immunogen affinity purified
Purity
≥95% as determined by SDS-PAGE
Immunogen
kelch-like ECH-associated protein 1
Recommended dilution
WB: 1:500 - 1:2000; IHC: 1:50 - 1:200; IF: 1:50 - 1:200
Synonyms
Cytosolic inhibitor of Nrf2, INrf2, KEAP1, Kelch like protein 19, KIAA0132, KLHL19
Image1
Immunohistochemistry of paraffin-embedded human lung cancer using FNab04516(KEAP1 antibody) at dilution of 1:100
Storage
PBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20℃ for 24 months (Avoid repeated freeze / thaw cycles.)
Image4
HepG2 cells were subjected to SDS PAGE followed by western blot with FNab04516(KEAP1 antibody) at dilution of 1:1000
Properties
If you buy Antibodies supplied by FineTest they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.Antibody for research use.
Background
This gene encodes a protein containing KELCH-1 like domains, as well as a BTB/POZ domain. Kelch-like ECH-associated protein 1 interacts with NF-E2-related factor 2 in a redox-sensitive manner and the dissociation of the proteins in the cytoplasm is followed by transportation of NF-E2-related factor 2 to the nucleus. This interaction results in the expression of the catalytic subunit of gamma-glutamylcysteine synthetase. Two alternatively spliced transcript variants encoding the same isoform have been found for this gene.
