Specificity
Human
Uniprot ID
Q14676
Form
liquid
Calculated MW
270 kDa
French translation
anticorps
Tested Application
ELISA, IHC, WB
Synonyms
KIAA0170, NFBD1
Purification
Immunogen affinity purified
Purity
≥95% as determined by SDS-PAGE
Immunogen
mediator of DNA damage checkpoint 1
Recommended dilution
WB: 1:500 - 1:2000; IHC: 1:100 - 1:200
Storage
PBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20℃ for 24 months (Avoid repeated freeze / thaw cycles.)
Properties
If you buy Antibodies supplied by FineTest they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.Antibody for research use.
Background
The protein encoded by this gene contains an N-terminal forkhead domain, two BRCA1 C-terminal (BRCT) motifs and a central domain with 13 repetitions of an approximately 41-amino acid sequence. The encoded protein is required to activate the intra-S phase and G2/M phase cell cycle checkpoints in response to DNA damage. This nuclear protein interacts with phosphorylated histone H2AX near sites of DNA double-strand breaks through its BRCT motifs, and facilitates recruitment of the ATM kinase and meiotic recombination 11 protein complex to DNA damage foci.
