Uniprot ID
P51608
Calculated MW
83 kDa
Form
liquid
French translation
anticorps
Specificity
Human, Mouse
Tested Application
ELISA, IHC, WB
Purification
Immunogen affinity purified
Purity
≥95% as determined by SDS-PAGE
Recommended dilution
WB: 1:500 - 1:1000; IHC: 1:50 - 1:100
Immunogen
methyl CpG binding protein 2 (Rett syndrome)
Storage
PBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20℃ for 24 months (Avoid repeated freeze / thaw cycles.)
Image1
Immunohistochemistry of paraffin-embedded human prostate cancer using FNab05082(MECP2 antibody) at dilution of 1:50
Image4
MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with FNab05082( MECP2 Antibody) at dilution of 1:1000
Properties
If you buy Antibodies supplied by FineTest they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.Antibody for research use.
Background
DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MECP2, MBD1 and MBD2 can also repress transcription from methylated gene promoters. In contrast to other MBD family members, MECP2 is X-linked and subject to X inactivation. MECP2 is dispensible in stem cells, but is essential for embryonic development. MECP2 gene mutations are the cause of most cases of Rett syndrome, a progressive neurologic developmental disorder and one of the most common causes of mental retardation in females. Alternative splicing results in multiple transcript variants encoding different isoforms.
