Specificity
Human
Uniprot ID
P52815
Calculated MW
21 kDa
Synonyms
RPML12
Form
liquid
French translation
anticorps
Tested Application
ELISA, WB, IHC
Purification
Immunogen affinity purified
Purity
≥95% as determined by SDS-PAGE
Immunogen
mitochondrial ribosomal protein L12
Recommended dilution
WB: 1:500 - 1:2000; IHC: 1:50 - 1:100
Storage
PBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20℃ for 24 months (Avoid repeated freeze / thaw cycles.)
Image1
Immunohistochemistry of paraffin-embedded human colon cancer using FNab05315(MRPL12 antibody) at dilution of 1:100
Image4
HeLa cells were subjected to SDS PAGE followed by western blot with FNab05315(MRPL12 antibody) at dilution of 1:1000
Properties
If you buy Antibodies supplied by FineTest they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.Antibody for research use.
Background
Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein which forms homodimers. In prokaryotic ribosomes, two L7/L12 dimers and one L10 protein form the L8 protein complex.
