anti- NEDD4L antibody

Size

2X100μg

Catalog no#

FNab05646

Price

320 EUR

Uniprot ID

Q96PU5

Form

liquid

Calculated MW

113 kDa

French translation

anticorps

Tested Application

ELISA, WB, IHC

Synonyms

KIAA0439, NEDL3

Specificity

Human, Mouse, Rat

Purification

Immunogen affinity purified

Purity

≥95% as determined by SDS-PAGE

Recommended dilution

WB: 1:500 - 1:2000; IHC: 1:50 - 1:200

Immunogen

neural precursor cell expressed, developmentally down-regulated 4-like

Storage

PBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20℃ for 24 months (Avoid repeated freeze / thaw cycles.)

Image4

K-562 cells were subjected to SDS PAGE followed by western blot with FNab05646(NEDD4L antibody) at dilution of 1:1000

Image1

Immunohistochemistry of paraffin-embedded human prostate cancer using FNab05646(NEDD4L antibody) at dilution of 1:100

Properties

If you buy Antibodies supplied by FineTest they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.

Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.Antibody for research use.

Background

This gene encodes a member of the Nedd4 family of HECT domain E3 ubiquitin ligases. HECT domain E3 ubiquitin ligases transfer ubiquitin from E2 ubiquitin-conjugating enzymes to protein substrates, thus targeting specific proteins for lysosomal degradation. The encoded protein mediates the ubiquitination of multiple target substrates and plays a critical role in epithelial sodium transport by regulating the cell surface expression of the epithelial sodium channel, ENaC. Single nucleotide polymorphisms in this gene may be associated with essential hypertension. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.