Uniprot ID
Q02218
Form
liquid
Calculated MW
116 kDa
French translation
anticorps
Specificity
Human, Mouse, Rat
Tested Application
ELISA, WB, IHC, IF
Purification
Immunogen affinity purified
Purity
≥95% as determined by SDS-PAGE
Recommended dilution
WB: 1:500 - 1:2000; IHC: 1:50 - 1:200; IF: 1:50 - 1:100
Immunogen
oxoglutarate (alpha-ketoglutarate) dehydrogenase (lipoamide)
Image1
Immunohistochemistry of paraffin-embedded human lung cancer using FNab05976(OGDH antibody) at dilution of 1:100
Storage
PBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20℃ for 24 months (Avoid repeated freeze / thaw cycles.)
Image4
mouse lung tissue were subjected to SDS PAGE followed by western blot with FNab05976( OGDH Antibody) at dilution of 1:10000
Properties
If you buy Antibodies supplied by FineTest they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.Antibody for research use.
Background
This gene encodes one subunit of the 2-oxoglutarate dehydrogenase complex. This complex catalyzes the overall conversion of 2-oxoglutarate (alpha-ketoglutarate) to succinyl-CoA and CO(2) during the Krebs cycle. The protein is located in the mitochondrial matrix and uses thiamine pyrophosphate as a cofactor. A congenital deficiency in 2-oxoglutarate dehydrogenase activity is believed to lead to hypotonia, metabolic acidosis, and hyperlactatemia. Alternative splicing results in multiple transcript variants encoding distinct isoforms.
