Synonyms
DAN
Specificity
Human
Uniprot ID
O95453
Calculated MW
75 kDa
Form
liquid
French translation
anticorps
Tested Application
ELISA, WB, IHC
Purification
Immunogen affinity purified
Purity
≥95% as determined by SDS-PAGE
Recommended dilution
WB: 1:1000 - 1:3000; IHC: 1:50 - 1:200
Immunogen
poly(A)-specific ribonuclease (deadenylation nuclease)
Storage
PBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20℃ for 24 months (Avoid repeated freeze / thaw cycles.)
Image1
Immunohistochemistry of paraffin-embedded human endometrial cancer using FNab06150(PARN antibody) at dilution of 1:100
Image4
MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with FNab06150(PARN antibody) at dilution of 1:1000
Properties
If you buy Antibodies supplied by FineTest they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.Antibody for research use.
Background
The protein encoded by this gene is a 3'-exoribonuclease, with similarity to the RNase D family of 3'-exonucleases. It prefers poly(A) as the substrate, hence, efficiently degrades poly(A) tails of mRNAs. Exonucleolytic degradation of the poly(A) tail is often the first step in the decay of eukaryotic mRNAs. This protein is also involved in silencing of certain maternal mRNAs during oocyte maturation and early embryonic development, as well as in nonsense-mediated decay (NMD) of mRNAs that contain premature stop codons. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
