Uniprot ID
P54727
Calculated MW
60 kDa
Form
liquid
French translation
anticorps
Specificity
Human, Mouse
Tested Application
ELISA, WB, IHC
Purification
Immunogen affinity purified
Immunogen
RAD23 homolog B (S. cerevisiae)
Purity
≥95% as determined by SDS-PAGE
Recommended dilution
WB: 1:500 - 1:2000; IHC: 1:100 - 1:200
Storage
PBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20℃ for 24 months (Avoid repeated freeze / thaw cycles.)
Image1
Immunohistochemistry of paraffin-embedded human breast cancer using FNab07078(RAD23B antibody) at dilution of 1:50
Image4
Jurkat cells were subjected to SDS PAGE followed by western blot with FNab07078(RAD23B antibody) at dilution of 1:1000
Properties
If you buy Antibodies supplied by FineTest they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.Antibody for research use.
Background
The protein encoded by this gene is one of two human homologs of Saccharomyces cerevisiae Rad23, a protein involved in the nucleotide excision repair (NER). This protein was found to be a component of the protein complex that specifically complements the NER defect of xeroderma pigmentosum group C (XP-c) cell extracts in vitro. This protein was also shown to interact with, and elevate the nucleotide excision activity of 3-methyladenine-DNA glycosylase (MPG), which suggested a role in DNA damage recognition in base excision repair. This protein contains an N-terminal ubiquitin-like domain, which was reported to interact with 26S proteasome, and thus this protein may be involved in the ubiquitin mediated proteolytic pathway in cells. Alternative splicing results in multiple transcript variants encoding distinct isoforms.
