Uniprot ID
P49459
Form
liquid
French translation
anticorps
Tested Application
ELISA, WB, IHC
Calculated MW
Refer to figures
Specificity
Human, Mouse, Rat
Purification
Immunogen affinity purified
Purity
≥95% as determined by SDS-PAGE
Recommended dilution
WB: 1:500 - 1:2000; IHC: 1:50 - 1:100
Immunogen
ubiquitin-conjugating enzyme E2A (RAD6 homolog)
Synonyms
HHR6A, HR6A, RAD6 homolog A, RAD6A, UBC2, UBE2A, Ubiquitin carrier protein A, Ubiquitin protein ligase A
Image1
Immunohistochemistry of paraffin-embedded human lymphoma using FNab09163(UBE2A antibody) at dilution of 1:50
Storage
PBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20℃ for 24 months (Avoid repeated freeze / thaw cycles.)
Image4
HEK-293 cells were subjected to SDS PAGE followed by western blot with FNab09163(UBE2A antibody) at dilution of 1:1000
Properties
If you buy Antibodies supplied by FineTest they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.Antibody for research use.
Background
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, ubiquitin-conjugating enzymes, and ubiquitin-protein ligases. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is required for post-replicative DNA damage repair, and may play a role in transcriptional regulation. Mutations in this gene are associated with mental retardation. Alternative splicing results in multiple transcript variants.