Uniprot ID
P51668
Calculated MW
14 kDa
Form
liquid
French translation
anticorps
Tested Application
ELISA, WB, IHC
Specificity
Human, Mouse, Rat
Purification
Immunogen affinity purified
Purity
≥95% as determined by SDS-PAGE
Recommended dilution
WB: 1:500 - 1:2000; IHC: 1:50 - 1:200
Immunogen
ubiquitin-conjugating enzyme E2D 1 (UBC4/5 homolog, yeast)
Storage
PBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20℃ for 24 months (Avoid repeated freeze / thaw cycles.)
Image1
Immunohistochemistry of paraffin-embedded human prostate cancer using FNab09167(UBE2D1 antibody) at dilution of 1:50
Image4
mouse thymus tissue were subjected to SDS PAGE followed by western blot with FNab09167(UBE2D1 antibody) at dilution of 1:1000
Properties
If you buy Antibodies supplied by FineTest they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
Synonyms
E2(17)KB1, SFT, Stimulator of Fe transport, UBC4/5, UBC4/5 homolog, UBC5A, UBCH5, UBCH5A, UBE2D1, Ubiquitin carrier protein D1, Ubiquitin protein ligase D1
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.Antibody for research use.
Background
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is closely related to a stimulator of iron transport (SFT), and is up-regulated in hereditary hemochromatosis. It also functions in the ubiquitination of the tumor-suppressor protein p53 and the hypoxia-inducible transcription factor HIF1alpha by interacting with the E1 ubiquitin-activating enzyme and the E3 ubiquitin-protein ligases. Two transcript variants encoding different isoforms have been found for this gene.