Antibody's target
CD9
Gene number
928
Total weight (kg)
1.4
Net weight (g)
0.1
Country of production
Canada
Protein number
P21926
Storage recommendations
-20°C
Raised in
Rabbit
Antibody in cell
Membrane
Stock availabilit
In Stock
Released date
6/Sep/2016
NCBI number
NP_001760.1
Conjugation
Biotinylated
Tested reactivity
To be tested
Tested applications
To be tested
Warning information
Non-hazardous
PubMed number
Refer to PubMed
Verified applications
WB, IHC, ICC/IF
Shipping recommendations
Blue Ice or 4°C
Representative figure link
No Data Available
Antibody's reactivity
Human, Mouse, Rat
Antibody's category
Polyclonal Antibodies
Latin name
Oryctolagus cuniculus
Immunogen
A synthesized peptide
Antibody's purified from
Peptide Affinity Purified
Group
Polyclonals and antibodies
Antibody's full description
Rabbit Anti- CD9 Polyclonal
Antibody's specificity
Detects endogenous levels of total CD9
Antibody's concentration
See included datasheet or contact our support service
Recommended buffer for storage
PBS pH 7.4, 50% glycerol, 150mM NaCl, 0.02% sodium azide
Tissue specificity
Expressed by a variety of hematopoietic and epithelial cells.
Antibody's datasheet
Contact our support service to receive datasheet or other technical documentation.
Primary research fields
Immunology, Cell Type Markers, CD Markers, Stem Cells, Embryonic Stem Cells, ESC Surface Molecules
Antibody's recommended dilutions for use
WB (1:1000), IHC (1:200), ICC/IF (1:200); optimal dilutions for assays should be determined by the user.
Certificate of analysis
A 1:1000 dilution of SPC-1272 was sufficient for detection of CD9 in 10 µg of COLO cell lysates by ECL immunoblot analysis using Goat Anti-Rabbit IgG:HRP as the secondary antibody.
Bibliography
1. Miao W.M., et al. (2001) Blood. 97:1689. 2. Ellerman D.A., et al. (2003) Mol. Biol Cell. 14: 5098-5103. 3. Schlossman S., et al. (1995) Leucocyte Typing V. Oxford University Press. New York.
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.Antibody for research use.
Properties
Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
Antibody's other name
CD9 antigen Antibody, CD9 antigen p24 Antibody, Leukocyte antigen MIC3 Antibody, Motility-related protein Antibody, Tetraspanin-29 Antibody, Tspan-29 Antibody, TSPAN29 Antibody, BA-2/p24 antigen Antibody, BA2 Antibody, Cell growth inhibiting gene 2 protein Antibody, DRAP 27 Antibody, GIG2 Antibody, Growth inhibiting gene 2 protein Antibody
Scientific context
CD9 is a 24 kD type III transmembrane protein also known as tetraspanin, MRP-1 and DRAP-24. It is a member of the tetraspan family (spanning the membrane four times) found on platelets, B cell progenitors, activated lymphocytes, granulocytes, endothelial cells and epithelial cells. CD9 induces adhesion, platelet aggregation, and B cell development. CD9 has been shown to associate with CD63, CD81, CD82, and CD36 and to bind to β1 integrins. It plays a role in cell signaling by mediating interactions with low molecular weight GTP-binding proteins.
About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
