Mouse Anti- Acetylated Lysine Monoclonal IgG1, Clone 7F8

Size

100 µg

Catalog no#

SMC-153D-HRP

Price

384 EUR

Clone

7F8

Net weight (g)

0.1

Total weight (kg)

1.4

Brandname

None

Additional isotype

IgG1

Raised in

Mouse

Country of production

Canada

Storage recommendations

-20ºC

Antibody's concentration

1 mg/ml

Antibody in cell

Nucleus

Stock availability

In Stock

Released date

1-Nov-2007

Tested applications

To be tested

Tested reactivity

To be tested

Latin name

Mus musculus

NCBI number

Refer to NCBI

Warning information

Non-hazardous

Immunogen

Acetylated KLH

Gene number

Refer to GenBank

Shipping recommendations

Blue Ice or 4ºC

Antibody's target

Acetylated Lysine

Purity

Protein G Purified

Protein number

Refer to Swiss-Prot

Antibody's category

Monoclonal Antibodies

Antibodies' applications

WB, IHC, ICC/IF, IP, ELISA

Antibody's datasheet

Contact our support service

Antibody buffer for storage

PBS pH7.4, 50% glycerol, 0.09% sodium azide

Tissue specificity

See included datasheet or contact our support service

Primary research fields

Cell Signaling, Post-translational Modifications, Acetylation

Antibody's reactivity

Species Independent. Bovine albumin and avian histones were tested.

Antibody's other name

Acetyl Lysine Antibody, Acetylated lysine Antibody, Lysine Antibody

Antibody's full description

Mouse Anti- Acetylated Lysine Monoclonal IgG1 Antibody, Clone: 7F8: HRP

Antibody's dilutions

WB (1:1000), IHC (1:100); optimal dilutions for assays should be determined by the user.

Antibody's specificity

Detects proteins containing acetylated lysine residues in ELISA and western blots. Does not detect non-acetylated lysine residues.

Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.Antibody for research use.

Antibody certificate of analysis

1 µg of SMC-153 was sufficient to detect acetylated chicken erythrocyte histones (sodium butyrate-treated) using 20 µg total protein, on western blot by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

About

Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.

Bibliography

1. Yang XJ. (2005). Oncogene. 24:1653-1662. 2. Hassig, C.A. and Schreiber, S.L. (1997). Curr. Opin. Chem. Biol. 1(3): 300-308. 3. Yang XJ. (2004). Bioessays 26:1076-1087. 4. Hughes, R.E. (2002). Curr. Biol. 12: R141-R143. 5. Vigushin, D.M. and Coombes, R.C. (2004). Curr. Cancer Drug Targets 4: 205-218.

Representative figure link

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Test

Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.

Scientific context

Post-translational modifications of proteins play critical roles in the regulation and function of many known biological processes. Proteins can be post-translationally modified in many different ways, and a common post-transcriptional modification of Lysine involves acetylation (1). The conserved amino-terminal domains of the four core histones (H2A, H2B, H3 and H4) contain lysines that are acetylated by histone acetyltransferases (HATs) and deacetylated by histone deacetylases (HDACs) (2). Protein posttranslational reversible lysine Nε development (5).

Representative figure legend

Immunohistochemistry analysis using Mouse Anti-Acetylated Lysine Monoclonal Antibody, Clone 7F8 (SMC-153). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Acetylated Lysine Monoclonal Antibody (SMC-153) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Western Blot analysis of acetylated lysine showing detection of Acetylated Lysine protein using Mouse Anti-Acetylated Lysine Monoclonal Antibody, Clone 7F8 (SMC-153). Primary Antibody: Mouse Anti-Acetylated Lysine Monoclonal Antibody (SMC-153) at 1:1000. (1) acetylated BSA (75ng of protein), (2) non-acetylated BSA, and (3) marker. Mouse Anti-Acetylated Lysine Antibody [7F8] used in Immunohistochemistry (IHC) on Mouse backskin (SMC-153) Mouse Anti-Acetylated Lysine Antibody [7F8] used in Western Blot (WB) on acetylated lysine (SMC-153)