Clone
7F8
Net weight (g)
0.1
Total weight (kg)
1.4
Brandname
None
Additional isotype
IgG1
Raised in
Mouse
Country of production
Canada
Storage recommendations
-20ºC
Antibody's concentration
1 mg/ml
Antibody in cell
Nucleus
Stock availability
In Stock
Released date
1-Nov-2007
Tested applications
To be tested
Tested reactivity
To be tested
Latin name
Mus musculus
NCBI number
Refer to NCBI
Warning information
Non-hazardous
Immunogen
Acetylated KLH
Gene number
Refer to GenBank
Shipping recommendations
Blue Ice or 4ºC
Antibody's target
Acetylated Lysine
Purity
Protein G Purified
Protein number
Refer to Swiss-Prot
Antibody's category
Monoclonal Antibodies
Antibodies' applications
WB, IHC, ICC/IF, IP, ELISA
Antibody's datasheet
Contact our support service
Antibody buffer for storage
PBS pH7.4, 50% glycerol, 0.09% sodium azide
Tissue specificity
See included datasheet or contact our support service
Primary research fields
Cell Signaling, Post-translational Modifications, Acetylation
Antibody's reactivity
Species Independent. Bovine albumin and avian histones were tested.
Antibody's other name
Acetyl Lysine Antibody, Acetylated lysine Antibody, Lysine Antibody
Antibody's full description
Mouse Anti- Acetylated Lysine Monoclonal IgG1 Antibody, Clone: 7F8: RPE
Antibody's dilutions
WB (1:1000), IHC (1:100); optimal dilutions for assays should be determined by the user.
Antibody's specificity
Detects proteins containing acetylated lysine residues in ELISA and western blots. Does not detect non-acetylated lysine residues.
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.Antibody for research use.
Antibody certificate of analysis
1 µg of SMC-153 was sufficient to detect acetylated chicken erythrocyte histones (sodium butyrate-treated) using 20 µg total protein, on western blot by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
About
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
Bibliography
1. Yang XJ. (2005). Oncogene. 24:1653-1662. 2. Hassig, C.A. and Schreiber, S.L. (1997). Curr. Opin. Chem. Biol. 1(3): 300-308. 3. Yang XJ. (2004). Bioessays 26:1076-1087. 4. Hughes, R.E. (2002). Curr. Biol. 12: R141-R143. 5. Vigushin, D.M. and Coombes, R.C. (2004). Curr. Cancer Drug Targets 4: 205-218.
Representative figure link
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
Scientific context
Post-translational modifications of proteins play critical roles in the regulation and function of many known biological processes. Proteins can be post-translationally modified in many different ways, and a common post-transcriptional modification of Lysine involves acetylation (1). The conserved amino-terminal domains of the four core histones (H2A, H2B, H3 and H4) contain lysines that are acetylated by histone acetyltransferases (HATs) and deacetylated by histone deacetylases (HDACs) (2). Protein posttranslational reversible lysine Nε development (5).
Representative figure legend
Immunohistochemistry analysis using Mouse Anti-Acetylated Lysine Monoclonal Antibody, Clone 7F8 (SMC-153). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Acetylated Lysine Monoclonal Antibody (SMC-153) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Western Blot analysis of acetylated lysine showing detection of Acetylated Lysine protein using Mouse Anti-Acetylated Lysine Monoclonal Antibody, Clone 7F8 (SMC-153). Primary Antibody: Mouse Anti-Acetylated Lysine Monoclonal Antibody (SMC-153) at 1:1000. (1) acetylated BSA (75ng of protein), (2) non-acetylated BSA, and (3) marker. Mouse Anti-Acetylated Lysine Antibody [7F8] used in Immunohistochemistry (IHC) on Mouse backskin (SMC-153) Mouse Anti-Acetylated Lysine Antibody [7F8] used in Western Blot (WB) on acetylated lysine (SMC-153)
