Mouse Anti- Phosphotyrosine Monoclonal IgG1 Kappa, Clone 13F9

Size

100 µl

Catalog no#

SMC-157D-A700

Price

365 EUR

Net weight (g)

0.1

Total weight (kg)

1.4

Clone

13F9

Brandname

None

Additional isotype

IgG1

Storage recommendations

4ºC

Raised in

Mouse

Country of production

Canada

Purity

Ascites

Stock availability

In Stock

Released date

1-Apr-2008

Tested applications

To be tested

Tested reactivity

To be tested

Latin name

Mus musculus

NCBI number

Refer to NCBI

Warning information

Non-hazardous

Antibody's target

Phosphotyrosine

Gene number

Refer to GenBank

Shipping recommendations

Blue Ice or 4ºC

Antibodies' applications

WB, ICC/IF, ELISA

Antibody's reactivity

Species Independent

Protein number

Refer to Swiss-Prot

Antibody's category

Monoclonal Antibodies

Antibody buffer for storage

Ascites, 0.02% sodium azide

Antibody's datasheet

Contact our support service

Immunogen

Phosphotyrosine conjugated to KLH

Tissue specificity

See included datasheet or contact our support service

Antibody in cell

See included datasheet or contact our support service

Antibody's other name

PhosphoTyrosine (pY) Antibody, PhosphoTyrosine (pY) Antibody

Primary research fields

Cell Signaling, Post-translational Modifications, Phosphorylation

Antibody's full description

Mouse Anti- Phosphotyrosine Monoclonal IgG1 Kappa Antibody, Clone: 13F9: ATTO 700

Antibody's dilutions

WB (1:100), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.

Antibody's specificity

Reacts with phosphotyrosine, and detects the presence of phosphotyrosine in both un-stimulated and stimulated cell lysates. Does not cross-react with phosphoserine or phosphothreonine.

Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.Antibody for research use.

About

Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.

Representative figure link

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Bibliography

1. Goto H. et al. (2005) Nature Cell Biology 8: 180-187. 2. Blume-Jensen P. and Hunter T. (2001) Nature 411:355-365. 3. Downward J. (2001) Nature 411: 759-762. 4. Pawson T. and Saxton T.M. (1999) Cell 97: 675-678. 5. Frackelton A.R. Jr., Ross A.H., and Eisen H.N. (1983) Mol Cell Biol. 3: 1343-1352. 6. Ross A.H., Baltimore D., and Eisen H.N. (1981) Nature 294: 654-656. 7. Ostrovsky PC. (1995) Genes Dev. 9(16): 2034-2041.

Test

Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.

Scientific context

Protein phosphorylation is an important posttranslational modification that serves many key functions to regulate a protein’s activity, localization, and protein-protein interactions. Phosphorylation is catalyzed by various specific protein kinases, which involves removing a phosphate group from ATP and covalently attaching it to to a recipient protein that acts as a substrate. Most kinases act on both serine and threonine; others act on tyrosine, and a number (dual specificity kinases) act on all three. Because phosphorylation can occur at multiple sites on any given protein, it can therefore change the function or localization of that protein at any time (1). Changing the function of these proteins has been linked to a number of diseases, including cancer, diabetes, heart disease, inflammation and neurological disorders (2-4). In particular, the phosphorylation of tyrosine is considered one of the key steps in signal transduction and regulation of enzymatic activity (5). Phosphotyrosine can be detected through specific antibodies, and are helpful in facilitating the identification of tyrosine kinase substrates (6).

Representative figure legend

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Phosphotyrosine Monoclonal Antibody, Clone 13F9 (SMC-157). Tissue: HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Mouse Anti-Phosphotyrosine Monoclonal Antibody (SMC-157) at 1:100 for 12 hours at 4°C. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Diffuse nuclear and cytoplasmic staining. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-Phosphotyrosine Antibody. (C) Composite. Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Phosphotyrosine Monoclonal Antibody, Clone 13F9 (SMC-157). Tissue: HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Mouse Anti-Phosphotyrosine Monoclonal Antibody (SMC-157) at 1:100 for 12 hours at 4°C. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Diffuse nuclear and cytoplasmic staining. Magnification: 20x. (A) DAPI (blue) nuclear stain. (B) Anti-Phosphotyrosine Antibody. (C) Composite. Mouse Anti-Phosphotyrosine Antibody [13F9] used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human HeLa Cells (SMC-157) Mouse Anti-Phosphotyrosine Antibody [13F9] used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human HeLa Cells (SMC-157)