Net weight (g)
0.1
Total weight (kg)
1.4
Clone
13F9
Brandname
None
Additional isotype
IgG1
Storage recommendations
4ºC
Raised in
Mouse
Country of production
Canada
Purity
Ascites
Stock availability
In Stock
Released date
1-Apr-2008
Tested applications
To be tested
Tested reactivity
To be tested
Latin name
Mus musculus
NCBI number
Refer to NCBI
Warning information
Non-hazardous
Antibody's target
Phosphotyrosine
Gene number
Refer to GenBank
Shipping recommendations
Blue Ice or 4ºC
Antibodies' applications
WB, ICC/IF, ELISA
Antibody's reactivity
Species Independent
Protein number
Refer to Swiss-Prot
Antibody's category
Monoclonal Antibodies
Antibody buffer for storage
Ascites, 0.02% sodium azide
Antibody's datasheet
Contact our support service
Immunogen
Phosphotyrosine conjugated to KLH
Tissue specificity
See included datasheet or contact our support service
Antibody in cell
See included datasheet or contact our support service
Antibody's other name
PhosphoTyrosine (pY) Antibody, PhosphoTyrosine (pY) Antibody
Primary research fields
Cell Signaling, Post-translational Modifications, Phosphorylation
Antibody's full description
Mouse Anti- Phosphotyrosine Monoclonal IgG1 Kappa Antibody, Clone: 13F9: FITC
Antibody's dilutions
WB (1:100), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
Antibody's specificity
Reacts with phosphotyrosine, and detects the presence of phosphotyrosine in both un-stimulated and stimulated cell lysates. Does not cross-react with phosphoserine or phosphothreonine.
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.Antibody for research use.
About
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
Representative figure link
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Bibliography
1. Goto H. et al. (2005) Nature Cell Biology 8: 180-187. 2. Blume-Jensen P. and Hunter T. (2001) Nature 411:355-365. 3. Downward J. (2001) Nature 411: 759-762. 4. Pawson T. and Saxton T.M. (1999) Cell 97: 675-678. 5. Frackelton A.R. Jr., Ross A.H., and Eisen H.N. (1983) Mol Cell Biol. 3: 1343-1352. 6. Ross A.H., Baltimore D., and Eisen H.N. (1981) Nature 294: 654-656. 7. Ostrovsky PC. (1995) Genes Dev. 9(16): 2034-2041.
Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
Scientific context
Protein phosphorylation is an important posttranslational modification that serves many key functions to regulate a protein’s activity, localization, and protein-protein interactions. Phosphorylation is catalyzed by various specific protein kinases, which involves removing a phosphate group from ATP and covalently attaching it to to a recipient protein that acts as a substrate. Most kinases act on both serine and threonine; others act on tyrosine, and a number (dual specificity kinases) act on all three. Because phosphorylation can occur at multiple sites on any given protein, it can therefore change the function or localization of that protein at any time (1). Changing the function of these proteins has been linked to a number of diseases, including cancer, diabetes, heart disease, inflammation and neurological disorders (2-4). In particular, the phosphorylation of tyrosine is considered one of the key steps in signal transduction and regulation of enzymatic activity (5). Phosphotyrosine can be detected through specific antibodies, and are helpful in facilitating the identification of tyrosine kinase substrates (6).
Representative figure legend
Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Phosphotyrosine Monoclonal Antibody, Clone 13F9 (SMC-157). Tissue: HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Mouse Anti-Phosphotyrosine Monoclonal Antibody (SMC-157) at 1:100 for 12 hours at 4°C. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Diffuse nuclear and cytoplasmic staining. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-Phosphotyrosine Antibody. (C) Composite. Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Phosphotyrosine Monoclonal Antibody, Clone 13F9 (SMC-157). Tissue: HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Mouse Anti-Phosphotyrosine Monoclonal Antibody (SMC-157) at 1:100 for 12 hours at 4°C. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Diffuse nuclear and cytoplasmic staining. Magnification: 20x. (A) DAPI (blue) nuclear stain. (B) Anti-Phosphotyrosine Antibody. (C) Composite. Mouse Anti-Phosphotyrosine Antibody [13F9] used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human HeLa Cells (SMC-157) Mouse Anti-Phosphotyrosine Antibody [13F9] used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human HeLa Cells (SMC-157)
