Net weight (g)
0.1
Total weight (kg)
1.4
Additional isotype
IgG1
Brandname
None
Raised in
Mouse
Country of production
Canada
Storage recommendations
-20ºC
Clone
3G4-1F5
Antibodies' applications
WB, IHC
Antibody's concentration
1 mg/ml
Antibody's target
Alginate
Stock availability
In Stock
Released date
24-Jun-2014
Tested applications
To be tested
Tested reactivity
To be tested
Latin name
Mus musculus
Warning information
Non-hazardous
NCBI number
Refer to NCBI
Gene number
Refer to GenBank
Shipping recommendations
Blue Ice or 4ºC
Purity
Protein G Purified
Antibody's reactivity
Species Independent
Protein number
Refer to Swiss-Prot
Antibody's category
Monoclonal Antibodies
Antibody's datasheet
Contact our support service
Immunogen
Sodium Alginate conjugated to KLH
Antibody buffer for storage
PBS pH7.4, 50% glycerol, 0.09% sodium azide
Primary research fields
Cell Signaling, Post-translational Modifications
Antibody in cell
See included datasheet or contact our support service
Tissue specificity
See included datasheet or contact our support service
Antibody's other name
Alginic Acid Antibody, Algin Antibody, Sodium Alginate Antibody
Antibody's dilutions
WB (1:500); optimal dilutions for assays should be determined by the user.
Antibody's specificity
Binds selectively to a BSA-conjugated alginate, but not to unconjugated BSA.
Antibody's full description
Mouse Anti- Alginate Monoclonal IgG1 Kappa Antibody, Clone: 3G4-1F5: ATTO 565
Antibody certificate of analysis
1 µg/ml of SMC-209 was sufficient for detection of 150 ng of alginate-conjugated BSA by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Bibliography
1. Zhai P., Chen X.B., and Schreyer D.J. (2013) Biofabrication. 5(1): 015009. 2. Selimoglu S.M., Ayyildiz-Tamis D., Gurhan I.D., and Elibol M. (2012) J Biosci Bioeng. 113(2):233-238.
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.Antibody for research use.
About
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
Scientific context
Sodium alginate is used in biological experiments for the immobilization of cells and encapsulation due to its biocompatibility and simple gelation with divalent cations such as Ca2+. Studies suggest that preparation of alginate microspheres will sustain protein delivery within tissue scaffolds (1), as well as in many other cell types (2).
Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
Representative figure legend
Western Blot analysis of ALL BSA-Alginate Conjugate showing detection of ~250 kDa Alginate protein using Mouse Anti-Alginate Monoclonal Antibody, Clone 3G4-1F5 (SMC-209). Lane 1: MW ladder. Lane 2: 0.625ug BSA:Alginate. Load: 0.625 µg. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Mouse Anti-Alginate Monoclonal Antibody (SMC-209) at 1:500 for 1 hour at RT. Secondary Antibody: HRP Goat Anti-Mouse at 1:100 for 1 hour at RT. Color Development: TMB solution for 2 min at RT. Predicted/Observed Size: ~250 kDa. Mouse Anti-Alginate Antibody [3G4-1F5] used in Western Blot (WB) on ALL BSA-Alginate Conjugate (SMC-209)
