Also known as
TNF
Host organism
N/A
Concentration
N/A
Tested applications:
N/A
Tissue
tumor
Category
Antibodies
Clonality
Monoclonal
Latin name
Mus musculus
Subcategory
Mnoclonal antibodies
Clone
2C8, 1D9, F6C5, TA20, TA40, TA70
Form/Appearance
PBS, pH 7.4, 0.1 % sodium azide (NaN3)
Storage and shipping
Store the antibody at +4 degrees Celsius.
Immunoglobulin isotype
IgG1 for MAbs 2C8, 1D9, F6C5, TA20, TA40, TA70
Other gene names
TNF; TNF; DIF; TNFA; TNFSF2; TNF-alpha; TNFA; TNFSF2; TNF-a; NTF; ICD1; ICD2
Gene name synonims
TNF; TNF; DIF; TNFA; TNFSF2; TNF-alpha; TNFA; TNFSF2; TNF-a; NTF; ICD1; ICD2
Gene name
TNF; TNF; DIF; TNFA; TNFSF2; TNF-alpha; TNFA; TNFSF2; TNF-a; NTF; ICD1; ICD2
Purification method
Chromatography on protein A Sepharose for MAbs 2C8, 1D9, F6C5, TA20, TA40, TA70
Species reactivity
N/A; Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.
About
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
Other names
TNF-alpha; Tumor necrosis factor; tumor necrosis factor; TNF-a; cachectin; APC1 protein; TNF, monocyte-derived; TNF, macrophage-derived; tumor necrosis factor-alpha; tumor necrosis factor ligand superfamily member 2; tumor necrosis factor; Cachectin; TNF-alpha; Tumor necrosis factor ligand superfamily member 2; TNF-a
Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
Specificity and cross-reactivity
MAb F6C5 is directed to different TNF-alpha epitope than MAbs 2C8 and 1D9. MAbs 2C8 and 1D9 are effective in inhibition of the biological activity of recombinant and natural TNF-alpha. MAbs TA20, TA40 and TA70 are specific to TNF-alpha. MAb TA20 has neutralizing activity.; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.
Description
The Monoclonal mouse anti- alpha tumor necrosis factor is a α- or alpha protein sometimes glycoprotein present in blood.This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.Antibody for research use.Aplha, transcription related growth factors and stimulating factors or repressing nuclear factors are complex subunits of proteins involved in cell differentiation. Complex subunit associated factors are involved in hybridoma growth, Eosinohils, eritroid proliferation and derived from promotor binding stimulating subunits on the DNA binding complex. NFKB 105 subunit for example is a polypetide gene enhancer of genes in B cells.
