Gene name synonims
N/A
Immunoglobulin isotype
IgG1
Also known as
CD276
Gene name
CD276
Clone
10B1218
Category
Antibodies
Clonality
Monoclonal
Tested applications:
Flow Cytometry
Conjugation
Anti-FITC Antibody
Subcategory
Mnoclonal antibodies
Concentration
0.1 miligram per 1 mililiter
Other gene names
CD276; CD276; B7H3; B7-H3; 4Ig-B7-H3; B7H3
Purification method
Purified by Protein G affinity chromatography.
Host organism
Mouse (Mus musculus) Source: Human (Homo sapiens)
Form/Appearance
Affinity Purified Supplied as a liquid in PBS, pH 7.4, 1% BSA, 0.09% sodium azide. Labeled with fluorescein isothiocyanate (FITC).
Species reactivity
Human (Homo sapiens); Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.
Other names
CD276 antigen isoform a; CD276 antigen; CD276 antigen; B7 homolog 3; OTTHUMP00000174913; OTTHUMP00000174914; costimulatory molecule; CD276 molecule; 4Ig-B7-H3; B7 homolog 3; B7-H3; Costimulatory molecule
Specificity and cross-reactivity
Recognizes human CD276.; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.Antibody for research use.Antigens are peptides or recombinant or native dependent on the production method.Whole adhesion and interacting molecules are present in lysates used as reference for ELISA quantification of these molecules and their subunits.
Storage and shipping
Store the antibody at +4 degrees Celsius for short term storage.. For long-term storage and to avoid repeated freezing and thawing, aliquot and For optimal long term storage, the antibody should be kept at -20 degrees Celsius. Aliquots are stable for at least 12 months the antibody should be stored at -20 degrees Celsius.. Prior to use, briefly centrifuge the original vial after thawing and before lid. If further dilution(s) are needed, they can be done with the appropriate assay buffer. FITC conjugates are sensitive to light.
Properties
This MBS Monoclonals Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MBS Monoclonals FITC is highly sensitive to pH extremes.
