Subcellular locations
N/A
Swiss Prot
N/A
Modification site
None
Gene ID
89870
Target Protein/Peptide
TRIM15
Applications
IF(IHC-P)
Excitation emission
590nm/617nm
Concentration
1ug per 1ul
Conjugated with
ALEXA FLUOR® 594
Conjugated
Alexa conjugate 1
Applications with corresponding dilutions
IF(IHC-P)(1:50-200)
Clonality
Polyclonal Antibody
Clone
Polyclonal Antibodies
Purification method
Purified by Protein A.
Other name
Anti- TRIM15 Polyclonal
Group
Polyclonals and antibodies
Also known as
TRIM15 Polyclonal Antibody
Type
Conjugated Primary Antibody
Conjugation
Alexa Fluor,ALEXA FLUOR® 594
Host organism
Rabbit (Oryctolagus cuniculus)
Properties
For facs or microscopy Alexa 1 conjugate.
Specificity
This antibody reacts specifically with TRIM15
Modification
No modification has been applied to this antibody
Antigen Source
KLH conjugated synthetic peptide derived from human TRIM15/RNF93
Cross reactive species
Human (Homo sapiens), Mouse (Mus musculus), Rat (Rattus norvegicus)
Storage
Water buffered solution containing 100ug/ml BSA, 50% glycerol and 0.09% sodium azide. Store at 4°C for 12 months.
Synonyms
RING finger protein 93; TRIM15; Tripartite mot containing 15; Tripartite mot protein 15; Tripartite mot-containing 15; Zinc finger protein 178; Zinc finger protein B7; ZNF178; ZNFB7; TRI15_HUMAN.
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.Antibody for research use.
About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.
Cross Reactive Species details
No significant cross reactivity has been observed for this antibody for the tested species. However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species.
Background information
The protein is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to the cytoplasm. Its function has not been identified. Alternate splicing of this gene results in two transcript variants encoding different isoforms.
Advisory
Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody, thus reducing its reactivity, specificity and sensitivity. For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube. Prior to use briefly centrifuge the vial to gather all the solution on the bottom.
