Subcellular locations
N/A
Swiss Prot
N/A
Gene ID
8440
Target Protein/Peptide
NCK2
Modification site
None
Applications
IF(IHC-P)
Excitation emission
590nm/617nm
Concentration
1ug per 1ul
Conjugated with
ALEXA FLUOR® 594
Conjugated
Alexa conjugate 1
Applications with corresponding dilutions
IF(IHC-P)(1:50-200)
Clonality
Polyclonal Antibody
Clone
Polyclonal Antibodies
Other name
Anti- NCK2 Polyclonal
Purification method
Purified by Protein A.
Also known as
NCK2 Polyclonal Antibody
Group
Polyclonals and antibodies
Type
Conjugated Primary Antibody
Conjugation
Alexa Fluor,ALEXA FLUOR® 594
Host organism
Rabbit (Oryctolagus cuniculus)
Properties
For facs or microscopy Alexa 1 conjugate.
Specificity
This antibody reacts specifically with NCK2
Modification
No modification has been applied to this antibody
Antigen Source
KLH conjugated synthetic peptide derived from human NCK2
Cross reactive species
Human (Homo sapiens), Mouse (Mus musculus), Rat (Rattus norvegicus)
Storage
Water buffered solution containing 100ug/ml BSA, 50% glycerol and 0.09% sodium azide. Store at 4°C for 12 months.
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.Antibody for research use.
About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.
Cross Reactive Species details
No significant cross reactivity has been observed for this antibody for the tested species. However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species.
Synonyms
Cytoplasmic protein NCK2; NCK2; GRB4; Growth factor receptor bound protein 4; NCK adaptor protein 2; Nck-2; NCK2; NCK2_HUMAN; NCKbeta; Non-catalytic region of tyrosine kinase adaptor protein 2; Noncatalytic region of tyrosine kinase beta; SH2/SH3 adaptor protein NCK-beta; SH2/SH3 adaptor protein NCK-beta.
Advisory
Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody, thus reducing its reactivity, specificity and sensitivity. For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube. Prior to use briefly centrifuge the vial to gather all the solution on the bottom.
Background information
This gene encodes a member of the NCK family of adaptor proteins. The protein contains three SH3 domains and one SH2 domain. The protein has no known catalytic function but has been shown to bind and recruit various proteins involved in the regulation of receptor protein tyrosine kinases. It is through these regulatory activities that this protein is believed to be involved in cytoskeletal reorganization. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq].
