Swiss Prot
N/A
Subcellular locations
N/A
Modification site
None
Gene ID
1663
Target Protein/Peptide
DDX11
Applications
IF(IHC-P)
Concentration
1ug per 1ul
Excitation emission
590nm/617nm
Conjugated with
ALEXA FLUOR® 594
Conjugated
Alexa conjugate 1
Applications with corresponding dilutions
IF(IHC-P)(1:50-200)
Clonality
Polyclonal Antibody
Cross reactive species
Human (Homo sapiens)
Clone
Polyclonal Antibodies
Other name
Anti- DDX11 Polyclonal
Purification method
Purified by Protein A.
Also known as
DDX11 Polyclonal Antibody
Group
Polyclonals and antibodies
Type
Conjugated Primary Antibody
Conjugation
Alexa Fluor,ALEXA FLUOR® 594
Host organism
Rabbit (Oryctolagus cuniculus)
Properties
For facs or microscopy Alexa 1 conjugate.
Specificity
This antibody reacts specifically with DDX11
Modification
No modification has been applied to this antibody
Antigen Source
KLH conjugated synthetic peptide derived from human DDX11
Storage
Water buffered solution containing 100ug/ml BSA, 50% glycerol and 0.09% sodium azide. Store at 4°C for 12 months.
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.Antibody for research use.
About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.
Cross Reactive Species details
No significant cross reactivity has been observed for this antibody for the tested species. However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species.
Advisory
Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody, thus reducing its reactivity, specificity and sensitivity. For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube. Prior to use briefly centrifuge the vial to gather all the solution on the bottom.
Synonyms
CHL1; CHL1 related helicase gene 1; CHL1-like helicase homolog; CHL1-related protein 1; CHLR1; Ddx11; DDX11_HUMAN; DEAD/H Asp Glu Ala Asp/His box polypeptide 11; DEAD/H box protein 11; hCHLR1; Keratinocyte growth factor regulated gene 2 protein; Keratinocyte growth factor-regulated gene 2 protein; KRG 2; KRG-2; KRG2; Probable ATP dependent RNA helicase DDX11; Probable ATP-dependent RNA helicase DDX11.
Background information
DNA helicase involved in cellular proliferation. Possesses DNA-dependent ATPase and helicase activities. This helicase translocates on single-stranded DNA in the 5' to 3' direction in the presence of ATP and, to a lesser extent, dATP. Its unwinding activity requires a 5'-single-stranded region for helicase loading, since flush-ended duplex structures do not support unwinding. The helicase activity is capable of displacing duplex regions up to 100 bp, which can be extended to 500 bp by RPA or the cohesion establishment factor, the Ctf18-RFC (replication factor C) complex activities. Stimulates the flap endonuclease activity of FEN1. Required for normal sister chromatid cohesion. Required for recruitement of bovine papillomavirus type 1 regulatory protein E2 to mitotic chrmosomes and for viral genome maintenance. Required for maintaining the chromosome segregation and is essential for embryonic development and the prevention of aneuploidy. May function during either S, G2, or M phase of the cell cycle. Binds to both single- and double-stranded DNA.Tissue specificity: Highly expressed in spleen, B-cells, thymus, testis, ovary, small intestine, and pancreas. Very low expression seen in the brain. Expressed in dividing cells and/or cells undergoing high levels of recombination. No expression is seen in cells signaled to terminally differentiate. Expressed in keratinocyte growth factor-stimulated cells but not in serum, EGF and IL1-beta-treated keratinocytes.
