Gene ID
8829
Modification site
None
Swiss Prot
O14786
Subcellular locations
Cytoplasm
Applications
IF(IHC-P)
Excitation emission
590nm/617nm
Concentration
1ug per 1ul
Target Protein/Peptide
Neuropilin 1
Conjugated
Alexa conjugate 1
Conjugated with
ALEXA FLUOR® 594
Applications with corresponding dilutions
IF(IHC-P)(1:50-200)
Clonality
Polyclonal Antibody
Clone
Polyclonal Antibodies
Purification method
Purified by Protein A.
Group
Polyclonals and antibodies
Type
Conjugated Primary Antibody
Other name
Anti- Neuropilin-1 Polyclonal
Conjugation
Alexa Fluor,ALEXA FLUOR® 594
Host organism
Rabbit (Oryctolagus cuniculus)
Also known as
Neuropilin-1 Polyclonal Antibody
Properties
For facs or microscopy Alexa 1 conjugate.
Modification
No modification has been applied to this antibody
Specificity
This antibody reacts specifically with Neuropilin-1
Antigen Source
KLH conjugated synthetic peptide derived from human neuropilin-1
Cross reactive species
Human (Homo sapiens), Mouse (Mus musculus), Rat (Rattus norvegicus)
Synonyms
NP1; NRP; BDCA4; CD304; VEGF165R; Neuropilin-1; Vascular endothelial cell growth factor 165 receptor; NRP1
Storage
Water buffered solution containing 100ug/ml BSA, 50% glycerol and 0.09% sodium azide. Store at 4°C for 12 months.
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.Antibody for research use.
About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.
Cross Reactive Species details
No significant cross reactivity has been observed for this antibody for the tested species. However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species.
Advisory
Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody, thus reducing its reactivity, specificity and sensitivity. For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube. Prior to use briefly centrifuge the vial to gather all the solution on the bottom.
Background information
The membrane-bound isoform 1 is a receptor involved in the development of the cardiovascular system, in angiogenesis, in the formation of certain neuronal circuits and in organogenesis outside the nervous system. It mediates the chemorepulsant activity of semaphorins. It binds to semaphorin 3A, The PLGF-2 isoform of PGF, The VEGF-165 isoform of VEGF and VEGF-B. Coexpression with KDR results in increased VEGF-165 binding to KDR as well as increased chemotaxis. It may regulate VEGF-induced angiogenesis. The soluble isoform 2 binds VEGF-165 and appears to inhibit its binding to cells. It may also induce apoptosis by sequestering VEGF-165. May bind as well various members of the semaphorin family. Its expression has an averse effect on blood vessel number and integrity.
