Anti- Complement C3, C3c Antibody

Size

1 ml

Catalog no#

GEN6004079

Price

746 EUR

French translation

anticorps

Category

Antibodies

Reacts with

Guinea Pig

Gene synonims

C3; C3; ASP

Applications

ELISA (EL/EIA)

Purification method

PurifiedPurified

Antigen

Complement C3, C3c

Gene name

Complement C3, C3c

Clonality

Polyclonal Antibodies

Long name

Complement C3, C3c (HRP)

Synonim names

Anti -Complement C3, C3c (HRP)

Clone

Not applicable to Polyclonal Antibodies

Concentration

Enquire us for detailed information on the concentration of this antibody

Other names

complement C3 preproprotein; Complement C3; complement C3; complement component 3

Properties

If you buy Antibodies supplied by MBS Polyclonals they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.

Form/Appearance

Supplied as a lyophilized powder from PBS, pH 7.2. Preservative free. Labeled with horseradish peroxidase (HRP). Reconstitute with 1ml sterile ddH2O. Dilute further in PBS, pH 7.2.

Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.Antibody for research use.

Specificity

Recognizes guinea pig serum Complement C3, C3c. Reacts with both native and activated C3. May also react with the fragments C3b, C3bi and C3dg, since they all carry antigenic epitopes of the C3c domain. In immunoelectrophoresis against fresh guinea pig serum, a single precipitin line is obtained in the beta-1 region representing native C3. Against serum containing partly activated C3, a precipitin line is obtained which extends from the beta-1 into the alpha-2 region, demonstrating a gradient. In old serum containing totally activated C3 a single precipitin line in the alpha-2 region is obtained. Also reacts with the fragments C3b, C3bi and smaller fragments, since they all carry antigenic determinants of the C3c domain. Does not react with any other ;This is an antibody designed to detect Complement C3, C3c

Storage, shipping and handling

Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. The antibody is shipped at +4 degrees Celsius. Upon receving, freeze at -20. For longer periods of time we recommend keeping the vial frozen at -40 or -80. Avoid cycles of freezing and thawing as they may denaturate the polypeptide chains of the antibody. Due to transportation or handling reasons, small amounts of the antibody might get caught on the lid or walls of the vial. Further dilutions can be made in assay buffer.  Labeled with horseradish peroxidase (HRP). Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. HRP conjugates are sensitive to light.