Mouse monoclonal-Anti- Ovalbumin (OVA)-20ug

Size

20ug

Catalog no#

MAB459Ge21-20ug

Price

253 EUR

Image number

1

Sequence of immunogen

-

Alternative Names

-

Aplication

WB

Concentration

1mg/ml

Species reactivity

Chicken

Item Name

Ovalbumin

Latin name

Mus musculus

Clonality

Mouse monoclonal

Delivery condition

4℃ with ice bags

Organism Species

Pan-species (General)

Source

Monoclonal antibody preparation

Immunogen

NPB459Ge01-Native Ovalbumin (OVA)

Purification

Protein A + Protein G affinity chromatography

Buffer Formulation

0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.

Applicable Secondary Antibody

SAA544Mu08, SAA544Mu09, SAA544Mu07, SAA544Mu19, SAA544Mu18, SAA544Mu17

Storage instructions

Avoid repeated freeze/thaw cycles. Store at 4 ℃ for frequent use. Aliquot and store at -20℃ for 12 months.

Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.Antibody for research use.

About

Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.

Test

Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.

Gene

Ovalbumin (abbreviated OVA) is the main protein found in egg white, making up 60-65% of the total protein. Ovalbumin displays sequence and three-dimensional homology to the serpin superfamily, but unlike most serpins it is not a serine protease inhibitor. The function of ovalbumin is unknown, although it is presumed to be a storage protein. OVA is also the best characterized and the first antigen proteins used as a transgene to make transgenic mice. Many different transgenic mouse models have systemic OVA expression driven by the ubiquitously expressed b-actin promoter or tissue-specific OVA expression with insulin promoter to drive the transgene expression, for studying type I diabetes, or in different isoforms, secreted or cell-membrane associated, and more recently as inducible transgene models. These C57BL/6 mice, BALB/c mice models are well characterized, and have contributed to our understanding of immunogenicity and tolerance by the OVA model.