Target of this antibody:
His
This antibody reacts with:
N/A
Clone:
RM146
Antibody host:
Rabbit
Form of antibody:
Liquid
Concentration of antibody:
1 mg/ml
Alternative names of antibody target:
His-Tag
Shipping conditions:
gel pack
French translation
anticorps
Conjugation
histidine
Isotype:
Rabbit IgG
Clonality of antibody:
Monoclonal
This antibody has been succesfully tested for use in following methods:
IP, WB, ICC, FC, IHC
Latin name
Oryctolagus cuniculus
Purification method:
Affinity purified using protein A
Storage information:
Shelf time: 12 months in temp.: -20 deg.C
Formulation of this antibody:
In 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
How to handle this antibody:
The antibody solution should be gently mixed before use.
Immunogene sequence:
Mixture of a peptide with 6xHis-Tag at the N-terminus and another peptide with 6xHis-Tag at the C-terminus.
Properties
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.Antibody for research use.
Recommended usage:
Western Blot: 0.1 ug/mL – 1 ug/mL;Immunoprecipitation: 0.5 ug/mL – 2 ug/mL;Immunocytochemistry 0.5 ug/mL – 2 ug/mL;Flow Cytometry: 0.5 ug/mL – 2 ug/mL;Immunohistochemistry; 0.1 ug/mL-1 ug/mL.
Background information:
A variety of plasmids contain DNA that encodes an N-terminal tag consisting of six histidine (His) residues, followed by an extended multiple cloning sites. The 6xHis tag on the expressed recombinant proteins allows for efficient coupling to Ni++ affinity resins and purification by a single step chromatography. As is the case with other protein tags, this polyhistidine tag can be cleaved at sites recognized by enzymes such as thrombin and enterokinases to isolate the protein of interest.
About
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.Rabbits are used for polyclonal antibody production by Biovision. Rabbit antibodies are very stable and can be stored for several days at room temperature. Biovision adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
