anti-Anti- Histone H2AZ, Rabbit Monoclonal Antibody

Size

Anti- Histone H2AZ, Rabbit Monoclonal Antibody

Catalog no#

A1142-25

Price

262 EUR

Target of this antibody:

H2AFZ

Clone:

RM215

This antibody reacts with:

Human

Antibody host:

Rabbit

Form of antibody:

Liquid

Concentration of antibody:

1 mg/ml

Shipping conditions:

gel pack

French translation

anticorps

Isotype:

Rabbit IgG

Clonality of antibody:

Monoclonal

Latin name

Oryctolagus cuniculus

This antibody has been succesfully tested for use in following methods:

WB, ICC, ELISA, Multiplex

Alternative names of antibody target:

Histone H2A.Z, H2A/z, H2AFZ

Purification method:

Affinity purified using protein A

Storage information:

Shelf time: 12 months in temp.: -20 deg.C

Formulation of this antibody:

In 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide

How to handle this antibody:

The antibody solution should be gently mixed before use.

Immunogene sequence:

A peptide corresponding to the C-terminus of human Histone H2AZ

Recommended usage:

WB: 0. 1 µg/mL – 0.5 µg/mL; ICC: 1 µg/mL - 2 µg/mL; ELISA: 0.1 µg/mL - 1 µg/mL; Multiplex: 0.1 µg/mL – 1 µg/mL.

Properties

If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.

Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.Antibody for research use.

Background information:

Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. May be involved in the formation of constitutive heterochromatin. May be required for chromosome segregation during cell division.

About

Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.Rabbits are used for polyclonal antibody production by Biovision. Rabbit antibodies are very stable and can be stored for several days at room temperature. Biovision adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.