anti- AIF antibody

Size

2X100μg

Catalog no#

FNab00235

Price

320 EUR

Uniprot ID

O95831

Calculated MW

67 kDa

Form

liquid

French translation

anticorps

Specificity

Human, Mouse

Synonyms

AIF, AIFM1, PDCD8

Tested Application

ELISA, IHC, WB, IF

Purification

Immunogen affinity purified

Purity

≥95% as determined by SDS-PAGE

Immunogen

apoptosis-inducing factor, mitochondrion-associated, 1

Recommended dilution

WB: 1:500 - 1:2000; IHC: 1:50 - 1:200; IF: 1:50 - 1:200

Storage

PBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20℃ for 24 months (Avoid repeated freeze / thaw cycles.)

Image4

Jurkat cells were subjected to SDS PAGE followed by western blot with FNab00235(AIF antibody) at dilution of 1:1000

Image1

Immunohistochemistry of paraffin-embedded human kidney tissue slide using FNab00235( AIF Antibody) at dilution of 1:100

Properties

If you buy Antibodies supplied by FineTest they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.

Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.Antibody for research use.

Background

This gene encodes a flavoprotein essential for nuclear disassembly in apoptotic cells, and it is found in the mitochondrial intermembrane space in healthy cells. Induction of apoptosis results in the translocation of this protein to the nucleus where it affects chromosome condensation and fragmentation. In addition, this gene product induces mitochondria to release the apoptogenic proteins cytochrome c and caspase-9. Mutations in this gene cause combined oxidative phosphorylation deficiency 6 (COXPD6), a severe mitochondrial encephalomyopathy, as well as Cowchock syndrome, also known as X-linked recessive Charcot-Marie-Tooth disease-4 (CMTX-4), a disorder resulting in neuropathy, and axonal and motor-sensory defects with deafness and mental retardation. Alternative splicing results in multiple transcript variants. A related pseudogene has been identified on chromosome 10.