anti- AMPK gamma 1 antibody

Size

2X100μg

Catalog no#

FNab00379

Price

320 EUR

Uniprot ID

P54619

Calculated MW

38 kDa

Form

liquid

French translation

anticorps

Tested Application

ELISA, WB, IHC

Specificity

Human, Mouse, Rat

Purification

Immunogen affinity purified

Purity

≥95% as determined by SDS-PAGE

Synonyms

AMPK gamma1, AMPK subunit gamma 1, AMPKG, PRKAG1

Recommended dilution

WB: 1:500 - 1:2000; IHC: 1:50 - 1:200; IF: 1:50 - 1:100

Immunogen

protein kinase, AMP-activated, gamma 1 non-catalytic subunit

Storage

PBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20℃ for 24 months (Avoid repeated freeze / thaw cycles.)

Image4

K-562 cells were subjected to SDS PAGE followed by western blot with FNab00379( PRKAG1 Antibody) at dilution of 1:1000

Image1

Immunohistochemistry of paraffin-embedded human testis tissue slide using FNab00379( PRKAG1 Antibody) at dilution of 1:50

Properties

If you buy Antibodies supplied by FineTest they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.

Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.Antibody for research use.

Background

The protein encoded by this gene is a regulatory subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. This subunit is one of the gamma regulatory subunits of AMPK. Alternatively spliced transcript variants encoding distinct isoforms have been observed.