anti- CDK4 antibody

Size

2X100μg

Catalog no#

FNab01557

Price

320 EUR

Uniprot ID

P11802

Calculated MW

32 kDa

Form

liquid

French translation

anticorps

Specificity

Human, Mouse, Rat

Tested Application

ELISA, WB, IHC, IF

Immunogen

cyclin-dependent kinase 4

Purification

Immunogen affinity purified

Purity

≥95% as determined by SDS-PAGE

Recommended dilution

WB: 1:500 - 1:2000; IHC: 1:50 - 1:200; IF: 1:20 - 1:100

Synonyms

CDK4, Cell division protein kinase 4, CMM3, cyclin dependent kinase 4, PSK J3

Storage

PBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20℃ for 24 months (Avoid repeated freeze / thaw cycles.)

Image4

HeLa cells were subjected to SDS PAGE followed by western blot with FNab01557(CDK4 antibody) at dilution of 1:1000

Image1

Immunohistochemistry of paraffin-embedded human lung cancer tissue slide using FNab01557( CDK4 Antibody) at dilution of 1:200

Properties

If you buy Antibodies supplied by FineTest they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.

Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.Antibody for research use.

Background

The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is highly similar to the gene products of S. cerevisiae cdc28 and S. pombe cdc2. It is a catalytic subunit of the protein kinase complex that is important for cell cycle G1 phase progression. The activity of this kinase is restricted to the G1-S phase, which is controlled by the regulatory subunits D-type cyclins and CDK inhibitor p16(INK4a). This kinase was shown to be responsible for the phosphorylation of retinoblastoma gene product (Rb). Mutations in this gene as well as in its related proteins including D-type cyclins, p16(INK4a) and Rb were all found to be associated with tumorigenesis of a variety of cancers. Multiple polyadenylation sites of this gene have been reported.