anti- EIF4G1 antibody

Size

2X100μg

Catalog no#

FNab02724

Price

320 EUR

Specificity

Human

Uniprot ID

Q04637

Form

liquid

Calculated MW

200 kDa

French translation

anticorps

Tested Application

ELISA, WB, IHC, IF

Synonyms

EIF4F, EIF4G, EIF4GI

Purification

Immunogen affinity purified

Purity

≥95% as determined by SDS-PAGE

Immunogen

eukaryotic translation initiation factor 4 gamma, 1

Recommended dilution

WB: 1:500 - 1:2000; IHC: 1:50 - 1:200; IF: 1:50 - 1:200

Storage

PBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20℃ for 24 months (Avoid repeated freeze / thaw cycles.)

Image1

Immunohistochemistry of paraffin-embedded human breast cancer using FNab02724(EIF4G1 antibody) at dilution of 1:100

Image4

HeLa cells were subjected to SDS PAGE followed by western blot with FNab02724(EIF4G1 antibody) at dilution of 1:1000

Properties

If you buy Antibodies supplied by FineTest they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.

Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.Antibody for research use.

Background

The protein encoded by this gene is a component of the multi-subunit protein complex EIF4F. This complex facilitates the recruitment of mRNA to the ribosome, which is a rate-limiting step during the initiation phase of protein synthesis. The recognition of the mRNA cap and the ATP-dependent unwinding of 5'-terminal secondary structure is catalyzed by factors in this complex. The subunit encoded by this gene is a large scaffolding protein that contains binding sites for other members of the EIF4F complex. A domain at its N-terminus can also interact with the poly(A)-binding protein, which may mediate the circularization of mRNA during translation. Alternative splicing results in multiple transcript variants, some of which are derived from alternative promoter usage.